The Orochem 96-Well Universal Cell Lysate Filter Plate (OC21DAHL / 64822-20) is designed for high-throughput clarification of bacterial, insect and human cell lysates before downstream protein isolation and characterisation. Its depth microfiltration architecture simultaneously removes cells, cell debris, insoluble precipitates, aggregates and colloidal material to produce clarified feed for subsequent processing.
Key Features
- 96-well high-throughput format, enabling parallel clarification of multiple cell lysate samples
- Designed for bacterial, insect and human cell lysates, supporting a broad range of protein-expression and sample-preparation workflows
- Depth microfiltration architecture, combining open-pore filtration with tighter filter-aid media
- Simultaneous removal of cells, debris and colloidal material, preparing cleaner feed for downstream processes
- Up to 800 µL sample volume per well, based on Orochem's official 96-well filtration procedure
- Rapid clarification workflow, with processing time reduced to approximately 5 minutes
- Low-speed centrifugation workflow, using 1000 × g for 2 minutes to collect clarified lysate
- Integrated filtration zones within each well, supporting staged depth filtration and particulate removal
- Good protein and sample-volume recovery, supporting efficient recovery of clarified lysate
- Performance comparable or superior to high-speed centrifugation, according to Orochem's cell-lysate clarification data
- Transparent, non-sterile plate
Benefits
- Clarify up to 96 cell lysate samples in parallel for higher-throughput protein workflows.
- Reduce reliance on lengthy high-speed centrifugation steps for routine lysate clarification.
- Remove larger cell debris and finer colloidal material within the same filtration device.
- Recover clarified lysate ready for downstream protein isolation, purification and characterisation.
- Use a standard centrifuge-based workflow requiring only 1000 × g for two minutes after sample loading.
Why Choose the Orochem 96-Well Cell Lysate Filter Plate (OC21DAHL / 64822-20)?
The OC21DAHL combines high-throughput 96-well processing with staged depth microfiltration to clarify bacterial, insect and human cell lysates efficiently. John Morris Group supplies the verified 64822-20 configuration for laboratories preparing clarified cell lysate before downstream protein purification and analytical workflows.
Recommended Centrifugation Workflow
- Perform lysate clarification at room temperature or 4°C
- Place the filter plate over a compatible collection plate
- Load up to 800 µL of cell lysate into each well
- Centrifuge the stacked plates at 1000 × g for 2 minutes to collect clarified lysate
