The Cytiva Superose 6 Increase 10/300 GL SEC Column, 24 mL (29091596) is a prepacked size exclusion chromatography column designed for high-resolution preparative purification, protein analysis and characterisation. Its Superose 6 Increase resin provides a broad globular protein fractionation range from Mr 5,000 to 5,000,000 and is particularly suited to large proteins, protein complexes and membrane proteins.
Key Features
- High-resolution size exclusion chromatography, designed for preparative purification, protein analysis and protein characterisation
- Broad globular protein fractionation range, covering molecular weights from Mr 5,000 to 5,000,000
- High-flow cross-linked agarose matrix, combining favourable pressure-flow properties with low nonspecific interaction
- Fine 8.6 µm average bead size, supporting high-resolution analytical separations
- 24 mL prepacked bed volume, with bed dimensions of 10 mm internal diameter × 300 mm height
- High column efficiency above 48,000 theoretical plates/m, supporting detailed separation of closely related molecular sizes
- Recommended sample volumes from 25 to 500 µL, suitable for microgram- to milligram-scale high-resolution SEC
- Recommended flow rate of 0.5 mL/min, with a maximum operating flow rate of 1.5 mL/min using water at 25°C
- Broad chemical operating range, with long-term pH stability from 3 to 12 and short-term stability from pH 1 to 14
- Operating temperature range of 4°C to 40°C, with storage recommended from 4°C to 30°C
- Designed for large biomolecules, including protein complexes, membrane proteins and other macromolecules
- Biocompatible column construction, with fittings provided for connection to ÄKTA chromatography systems or equivalent high-performance systems
Note: Flow rate should be reduced when operating at low temperatures or with viscous solutions.
Benefits
- Improves resolution compared with the previous Superose 6 generation, supporting higher-purity separations and improved analytical results.
- Supports rapid SEC workflows through the pressure-flow properties of the high-flow agarose matrix and small resin beads.
- Allows both preparative and analytical use, providing flexibility across protein purification, analysis and characterisation workflows.
- Supports recovery of biological materials through the resin's low nonspecific interaction.
- Withstands repeated high-pH cleaning procedures, helping minimise carry-over during repeated chromatography workflows.
Why Choose the Cytiva Superose 6 Increase 10/300 GL SEC Column, 24 mL (29091596)?
The Cytiva Superose 6 Increase 10/300 GL combines a broad molecular-weight fractionation range, high column efficiency and fine agarose resin for high-resolution separation of large proteins and other biomolecules. Buy from John Morris Group for Cytiva size exclusion chromatography columns and protein purification solutions in Australia.
