The Cytiva StrepTrap XT Prepacked Columns, 5 × 1 mL (29401320), are affinity chromatography columns designed for purification of Strep-tag II and Twin-Strep-tag recombinant proteins. Each 1 mL HiTrap-format column contains Strep-Tactin XT Sepharose and supports a typical loading of 10 mg of Strep-tag II protein per column.
Key Features
- Five prepacked 1 mL columns, supplied as a 5 × 1 mL configuration for repeat affinity purification workflows
- Designed for Strep-tag II protein purification, with Strep-Tactin XT ligand providing affinity capture of tagged recombinant proteins
- Suitable for Twin-Strep-tag proteins, extending use across common Strep-tag affinity purification workflows
- Typical loading of 10 mg per column for Strep-tag II protein in the 1 mL column format
- Recommended flow rate of 1 mL/min, with a maximum flow rate of 4 mL/min
- Strep-Tactin XT Sepharose resin, based on a rigid, cross-linked spherical agarose matrix
- Approximately 10 mg/mL dynamic binding capacity for Strep-tag II or Twin-Strep-tag protein, depending on the protein
- Operational pH range from 6 to 10, supporting common affinity purification conditions
- Fine 34 µm median particle size, supporting efficient chromatographic separation
- Higher-affinity XT chemistry, designed to support purification under denaturing conditions and minimise loss of target protein
Benefits
- Reduces preparation time by providing ready-to-use prepacked columns for Strep-tag affinity purification.
- Supports repeat experiments and multiple purification runs with five individual 1 mL columns in each pack.
- Provides strong affinity capture for Strep-tag II and Twin-Strep-tag proteins to support efficient recovery of valuable targets.
- Offers flexibility for purification workflows that require denaturing conditions.
- Provides convenient operation using a syringe with the supplied Luer adapter, a laboratory pump, or an ÄKTA liquid chromatography system.
Why Choose the Cytiva StrepTrap XT Prepacked Columns, 5 × 1 mL (29401320)?
The Cytiva StrepTrap XT 5 × 1 mL configuration combines Strep-Tactin XT Sepharose with a convenient prepacked HiTrap format for reproducible Strep-tagged protein purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
