The Cytiva Protein G Sepharose 4 Fast Flow Resin, 5 mL (17061801) is a bulk affinity chromatography medium for purification of immunoglobulins using recombinant Protein G coupled to Sepharose 4 Fast Flow. The 5 mL laboratory pack provides a total binding capacity of at least 20 mg human IgG per mL of resin.
Key Features
- Recombinant Protein G affinity ligand, produced in E. coli and engineered without the native albumin-binding region
- High IgG binding capacity, providing at least 20 mg human IgG per mL of resin under Cytiva's specified test conditions
- Broad antibody-binding capability, with Protein G binding IgG from a wide range of species and subclasses
- 5 mL bulk resin format, supplied for packing into suitable chromatography columns or use in batch purification workflows
- Cross-linked 4% spherical agarose matrix, providing the base structure for Protein G Sepharose 4 Fast Flow
- Approximately 90 µm median particle size, supporting Fast Flow chromatography operation
- Approximately 2 mg Protein G per mL of resin, providing the immobilised affinity ligand concentration
- Operational pH range from 3 to 9, supporting routine antibody affinity purification conditions
- CIP pH stability from 2 to 10, allowing cleaning and sanitisation within the specified range
- Pressure and flow characteristics of 150 to 250 cm/h, measured at less than 0.1 MPa under Cytiva's stated test conditions
- Suitable for immunoprecipitation workflows, in addition to packed-column antibody purification
- Supplied preswollen in ethanol, with recommended refrigerated storage between 2°C and 8°C
Note: Protein G Sepharose 4 Fast Flow must not be frozen.
Benefits
- Supports purification of a broad range of IgG antibodies through the binding characteristics of recombinant Protein G.
- Helps reduce unwanted albumin interaction because the recombinant ligand has been engineered without the native albumin-binding region.
- Provides flexibility for both packed-column chromatography and batch-based antibody purification workflows.
- Supports immunoprecipitation applications where selective capture of antibodies or antibody-associated targets is required.
- Offers a reusable chromatography medium that can be regenerated, cleaned and sanitised within the manufacturer's specified operating conditions.
Why Choose the Cytiva Protein G Sepharose 4 Fast Flow Resin, 5 mL (17061801)?
This 5 mL bulk affinity resin combines recombinant Protein G with a Fast Flow agarose matrix for flexible IgG purification, scale-up and immunoprecipitation workflows. Buy Cytiva Protein G Sepharose 4 Fast Flow from John Morris Group for antibody purification applications in Australia.
