The Cytiva PD MiniTrap G-25 Desalting Columns, Pack of 50 (28918007) are prepacked columns for desalting and buffer exchange of protein samples. Each column contains a 2.1 mL bed of Sephadex G-25 Medium and accommodates samples from 0.1 to 0.5 mL by gravity flow or 0.2 to 0.5 mL using the centrifugation protocol.
Key Features
- Prepacked with 2.1 mL of Sephadex G-25 Medium, providing a ready-to-use size exclusion medium for sample cleanup
- Handles 0.1 to 0.5 mL samples by gravity flow, supporting convenient small-volume desalting and buffer exchange
- Handles 0.2 to 0.5 mL samples by centrifugation, offering an alternative spin-based workflow
- Designed for proteins above Mr 5000, enabling removal of lower-molecular-weight substances from larger biomolecules
- Desalting capacity above 90%, supporting effective removal of low-molecular-weight components
- Protein recovery of 70 to 95%, depending on the biomolecule being processed
- Compact 0.97 × 2.8 cm packed bed, contained within a 5 mL column format
- Fifty prepacked columns per pack, supporting repeated sample preparation workflows
- Four centrifuge adapters included, providing the required adapters for the spin protocol
Benefits
- Perform desalting and buffer exchange without manually packing chromatography resin.
- Select gravity-flow or centrifugation protocols according to sample volume and workflow requirements.
- Process multiple samples conveniently using the simple gravity-flow method without a purification system.
- Minimise sample dilution when using the centrifugation protocol.
- Support routine protein sample preparation with 50 ready-to-use columns supplied in each pack.
Why Choose the Cytiva PD MiniTrap G-25 Desalting Columns, Pack of 50 (28918007)?
The Cytiva PD MiniTrap G-25 format combines a prepacked Sephadex G-25 Medium bed with flexible gravity-flow and centrifugation protocols for small-volume protein desalting and buffer exchange. Buy from John Morris Group for genuine Cytiva chromatography consumables for laboratory purification and sample preparation workflows.
