The Cytiva PD MidiTrap G-25 Desalting Columns, Pack of 50 (28918008) are prepacked columns designed for rapid and convenient cleanup of proteins and other large biomolecules above Mr 5,000. Each column contains a 3.5 mL bed of Sephadex G-25 resin and supports desalting, buffer exchange and removal of low-molecular-weight compounds using either gravity flow or centrifugation.
Key Features
- Designed for protein and biomolecule cleanup, including desalting, buffer exchange and removal of low-molecular-weight compounds
- 3.5 mL prepacked Sephadex G-25 bed, eliminating the need for manual resin preparation and packing
- 85–260 µm particle size range, providing the specified Sephadex G-25 resin format for group separation
- Handles 0.5–1.0 mL samples by gravity flow, enabling straightforward processing without a chromatography system
- Handles 0.75–1.0 mL samples by centrifugation, supporting rapid processing in a standard centrifuge
- Desalting capacity above 90%, supporting efficient removal of salts and other small molecules
- Mr 5,000 exclusion limit, separating larger biomolecules from lower-molecular-weight substances
- Typical recovery of 70–90%, depending on the protein being processed
- Working pH range from 2 to 13, allowing use with commonly used chromatography buffers
- Two operating protocols, providing a choice between simple gravity processing and centrifugation with minimal dilution
- Four centrifugation adapters included, allowing the supplied columns to be used with the spin protocol
Benefits
- Supports rapid sample cleanup without requiring a dedicated chromatography system when using the gravity protocol.
- Provides flexibility to choose gravity flow for simple parallel processing or centrifugation where minimal sample dilution is preferred.
- Accommodates sample volumes up to 1 mL, offering greater capacity than smaller G-25 spin-column formats.
- Provides defined desalting performance and recovery characteristics for routine protein sample preparation.
- Reduces preparation work through a ready-to-use prepacked format supplied in a 50-column pack.
Why Choose the Cytiva PD MidiTrap G-25 Desalting Columns, Pack of 50 (28918008)?
The Cytiva PD MidiTrap G-25 combines a 3.5 mL prepacked Sephadex G-25 bed with flexible gravity and centrifugation protocols for convenient desalting and buffer exchange of protein samples up to 1 mL. Buy from John Morris Group for Cytiva sample preparation and chromatography products in Australia.
