The Cytiva HiTrap SP HP Cation Exchange Columns, 5 × 1 mL (17115101) are prepacked ion exchange chromatography columns for high-resolution protein purification. Each 1 mL column contains SP Sepharose High Performance strong cation exchange resin with a 34 µm median particle size.
Key Features
- Strong cation exchange chemistry, using SP Sepharose High Performance resin for protein purification by charge
- Five prepacked 1 mL columns per pack, providing ready-to-use HiTrap columns for repeated purification workflows
- Typical loading range of 1–50 mg ribonuclease A per column, providing a defined reference capacity for the 1 mL format
- Recommended flow rate of 1 mL/min, with a maximum flow rate of 4 mL/min for the HiTrap 1 mL format
- Broad regular-use pH range from 4 to 13, supporting method development across varied buffer conditions
- Fine 34 µm median particle size, supporting high-resolution ion exchange separations
- Compact 7 × 25 mm packed-bed dimensions, providing a 1 mL chromatography bed in the HiTrap format
- Designed for convenient small-scale purification, with the HiTrap format suitable for routine ion exchange chromatography workflows
Benefits
- Perform high-resolution protein purification without manually packing an ion exchange column.
- Develop and optimise cation exchange methods across a broad pH range from 4 to 13.
- Balance resolution and throughput using recommended and maximum flow rates suited to the 1 mL format.
- Process repeated purification runs conveniently with five prepacked columns supplied in each pack.
- Use a strong cation exchanger when separating proteins according to differences in net surface charge.
Why Choose the Cytiva HiTrap SP HP Cation Exchange Columns, 5 × 1 mL (17115101)?
The Cytiva HiTrap SP HP combines SP Sepharose High Performance resin with a compact prepacked 1 mL format for high-resolution cation exchange chromatography. Buy from John Morris Group for genuine Cytiva chromatography columns and consumables for protein purification workflows.
