The Cytiva HiTrap Protein G HP Antibody Columns, 5 × 1 mL (17040401), are ready-to-use affinity chromatography columns for purification of IgG antibodies. Each 1 mL column is prepacked with Protein G Sepharose High Performance resin and provides an approximate total binding capacity of 25 mg human IgG per mL of resin.
Key Features
- Five prepacked 1 mL columns, supplied as a 5 × 1 mL configuration for repeat antibody purification workflows
- Designed for IgG antibody purification, using Protein G affinity chromatography for selective capture of immunoglobulins
- Protein G Sepharose High Performance resin, combining recombinant protein G ligand with a cross-linked agarose matrix
- Approximately 25 mg human IgG/mL resin total binding capacity, supporting practical small-scale antibody purification
- Fine 34 µm median particle size, providing the chromatographic characteristics of Sepharose High Performance resin
- Recommended operating flow rate of 1 mL/min, with a maximum operating flow rate of 4 mL/min for the 1 mL format
- Compact 1 mL column dimensions, with a 0.7 cm internal diameter and 2.5 cm bed height
- Column hardware rated to 5 bar, equivalent to 0.5 MPa or 70 psi
- Operational pH range from 3 to 9, with a cleaning-in-place pH range from 2 to 9
- Recombinant Protein G ligand, with the albumin-binding region of native Protein G genetically deleted
Benefits
- Reduces preparation time by providing ready-to-use prepacked affinity columns for routine antibody purification.
- Supports purification of polyclonal and monoclonal antibodies from cell culture supernatants, serum, and ascites.
- Provides broad IgG binding across a range of species and subclasses where Protein G affinity is suitable.
- Allows straightforward operation with a syringe, laboratory pump, or chromatography system.
- Supports repeat purification work with five individual 1 mL columns in each pack.
Why Choose the Cytiva HiTrap Protein G HP Antibody Columns, 5 × 1 mL (17040401)?
The Cytiva HiTrap Protein G HP 5 × 1 mL columns combine Protein G Sepharose High Performance resin with a convenient prepacked format for reproducible IgG antibody purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
