The Cytiva HiTrap Protein A HP Antibody Columns, 5 × 1 mL (17040201), are ready-to-use affinity chromatography columns for purification of antibodies from samples such as cell culture supernatants, serum, and ascites. Each 1 mL column is prepacked with Protein A Sepharose High Performance resin and provides an approximate total binding capacity of 20 mg human IgG per mL of resin.
Key Features
- Five prepacked 1 mL columns, supplied as a 5 × 1 mL configuration for repeat antibody purification workflows
- Designed for antibody purification, using Protein A affinity chromatography for capture of immunoglobulins
- Protein A Sepharose High Performance resin, providing a high-performance affinity medium for routine antibody purification
- Approximately 20 mg human IgG/mL resin total binding capacity, supporting practical small-scale purification workflows
- Fine 34 µm median particle size, helping minimise sample dilution in the eluted peak
- Recommended operating flow rate of 1 mL/min, with a maximum operating flow rate of 4 mL/min for the 1 mL format
- Compact 1 mL column dimensions, with a 0.7 cm internal diameter and 2.5 cm bed height
- Column hardware rated to 5 bar, equivalent to 0.5 MPa or 70 psi
- Operational pH range from 3 to 9, with a cleaning-in-place pH range from 2 to 10
Benefits
- Reduces preparation time by providing ready-to-use prepacked affinity columns for routine antibody purification.
- Supports purification of antibodies from common biological sample types including cell culture supernatants, serum, and ascites.
- Helps reduce dilution of the recovered antibody fraction through the small-particle High Performance resin format.
- Provides flexible operation using a syringe, laboratory pump, or chromatography system.
- Supports repeat purification work with five individual 1 mL columns in each pack.
Why Choose the Cytiva HiTrap Protein A HP Antibody Columns, 5 × 1 mL (17040201)?
The Cytiva HiTrap Protein A HP 5 × 1 mL columns combine Protein A Sepharose High Performance resin with a convenient prepacked format for routine antibody purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
