The Cytiva HiTrap Heparin HP Affinity Columns, 5 × 1 mL (17040601), are prepacked chromatography columns for high-resolution purification of biomolecules with affinity for heparin. Each 1 mL column is packed with Heparin Sepharose High Performance resin and provides a typical loading range of 0.2–3 mg bovine antithrombin III per column.
Key Features
- Five prepacked 1 mL columns, supplied as a 5 × 1 mL configuration for repeat affinity purification workflows
- Heparin Sepharose High Performance resin, using immobilised heparin as the affinity ligand
- Suitable for heparin-binding biomolecules, including DNA-binding proteins and other proteins with affinity for heparin
- Typical loading range of 0.2–3 mg per column, specified using bovine antithrombin III
- Approximately 3 mg/mL binding capacity, specified for bovine antithrombin III
- Recommended flow rate of up to 1 mL/min, with a maximum flow rate of 4 mL/min for the 1 mL format
- Fine 34 µm mean particle size, using highly cross-linked spherical agarose beads
- Stable across pH 5 to 10, for both long-term and short-term use according to Cytiva specifications
- Compact 1 mL column bed, measuring 7 mm internal diameter × 25 mm bed height
- Maximum pressure of 5 bar, equivalent to 0.5 MPa or 70 psi
Benefits
- Reduces column preparation requirements by providing ready-to-use prepacked affinity chromatography columns.
- Supports high-resolution purification of proteins and other biomolecules that interact with immobilised heparin.
- Provides repeatable small-scale purification across five individual 1 mL columns in each pack.
- Combines fine resin particles with practical flow rates for efficient laboratory chromatography workflows.
- Supports flexible method development across commonly used aqueous buffer systems.
Why Choose the Cytiva HiTrap Heparin HP Affinity Columns, 5 × 1 mL (17040601)?
The Cytiva HiTrap Heparin HP 5 × 1 mL columns combine Heparin Sepharose High Performance resin with a convenient prepacked format for purification of heparin-binding biomolecules. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
