The Cytiva HiTrap CM FF Cation Exchange Columns, 5 × 1 mL (17505601) are prepacked weak cation exchange chromatography columns for small-scale protein purification and screening of binding and elution conditions. Each 1 mL column contains CM Sepharose Fast Flow resin with a 90 µm median particle size and is designed for convenient ion exchange separations and method development.
Key Features
- Weak cation exchange chemistry, providing an alternative selectivity where a strong cation exchanger is not preferred
- CM Sepharose Fast Flow resin, using carboxymethyl functional groups on a cross-linked agarose matrix
- 90 µm median particle size, combining useful binding capacity with good flow properties for preparative ion exchange chromatography
- 1 mL prepacked bed volume, with five ready-to-use columns supplied in each pack
- Compact 7 × 25 mm packed bed, providing a convenient format for small-scale purification and method scouting
- Dynamic binding capacity of approximately 50 mg ribonuclease/mL resin, under Cytiva's specified test conditions
- Recommended flow rate up to 1 mL/min, with a maximum flow rate of 4 mL/min for the 1 mL HiTrap format
- Maximum packed-bed pressure of 0.3 MPa, defining the operating pressure limit for the prepacked column
- Operational pH stability from 4 to 13, with a recommended regular-use range of pH 6 to 10 for this CM Sepharose Fast Flow format
Benefits
- Reduces preparation time by providing factory-packed columns ready for cation exchange purification workflows.
- Provides weak cation exchange selectivity for proteins and other charged biomolecules where different selectivity from a strong exchanger is required.
- Supports rapid method development through a compact 1 mL format with defined flow, capacity and pressure characteristics.
- Offers straightforward scale-up within the Sepharose Fast Flow platform once suitable purification conditions have been established.
- Allows multiple columns to be used individually or connected in series to suit different small-scale purification requirements.
Why Choose the Cytiva HiTrap CM FF Cation Exchange Columns, 5 × 1 mL (17505601)?
The Cytiva HiTrap CM FF combines weak cation exchange selectivity with proven CM Sepharose Fast Flow resin in a convenient prepacked format for small-scale protein purification and method development. Buy from John Morris Group for Cytiva ion exchange chromatography columns and protein purification solutions in Australia.
