The Cytiva HisTrap HP His-Tag Protein Columns, 5 × 5 mL (17524802), are prepacked affinity chromatography columns for purification of His-tagged proteins. Each 5 mL column is packed with Ni Sepharose High Performance resin and provides a typical loading range of 5–200 mg of His-tagged protein per column.
Key Features
- Five prepacked 5 mL columns, supplied as a 5 × 5 mL pack for repeat protein purification workflows
- Designed for His-tagged protein purification, using immobilised metal ion affinity chromatography
- High-capacity Ni Sepharose High Performance resin, with a dynamic binding capacity of at least 40 mg of His-tagged protein per mL of resin
- Typical loading range of 5–200 mg per column for His-tagged protein purification
- Recommended flow rate of 5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL column format
- Broad working pH range from 3 to 12, supporting varied purification conditions
- Fine 34 µm resin particles, supporting high-resolution affinity chromatography
- Compact 5 mL HiTrap format, with a 1.6 cm internal diameter and 2.5 cm bed height
- Column hardware rated to 5 bar, equivalent to 0.5 MPa or 70 psi
Benefits
- Reduces column preparation time by providing prepacked chromatography columns ready for His-tag purification workflows.
- Supports higher sample loads than the 1 mL format, with a typical loading range extending to 200 mg per column.
- Provides repeatable purification capacity across five individual 5 mL columns in each pack.
- Combines high binding capacity with fine resin particles to support efficient capture and purification of target proteins.
- Offers compatibility with commonly used buffers, reducing agents, denaturants, and detergents for flexible method development.
Why Choose the Cytiva HisTrap HP His-Tag Protein Columns, 5 × 5 mL (17524802)?
The Cytiva HisTrap HP 5 × 5 mL configuration combines Ni Sepharose High Performance resin with a larger-capacity prepacked HiTrap format for convenient purification of His-tagged proteins. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
