The Cytiva HisTrap HP His-Tag Protein Columns, 5 × 1 mL (17524701), are prepacked affinity chromatography columns for purification of His-tagged proteins. Each 1 mL column is packed with Ni Sepharose High Performance resin and provides a typical loading range of 1–40 mg of His-tagged protein per column.
Key Features
- Five prepacked 1 mL columns, supplied in a 5 × 1 mL configuration for repeat purification workflows
- Designed for His-tagged protein purification, using immobilised metal ion affinity chromatography
- Ni Sepharose High Performance resin, providing the stationary phase for His-tag affinity purification
- Typical loading range of 1–40 mg per column for His-tagged protein
- Recommended flow rate of 1 mL/min, with a maximum flow rate of 4 mL/min
- Regular-use pH range from 3 to 12, supporting a broad range of purification conditions
- 34 µm median particle size for the chromatography resin
- HiTrap 1 mL column format, providing a compact prepacked format for laboratory protein purification
Benefits
- Reduces column preparation requirements by providing prepacked affinity chromatography columns ready for purification workflows.
- Supports multiple experiments or purification runs with five individual 1 mL columns in each pack.
- Provides useful binding capacity for small-scale His-tagged protein purification in a compact column format.
- Allows purification methods to be operated across a broad regular-use pH range.
Why Choose the Cytiva HisTrap HP His-Tag Protein Columns, 5 × 1 mL (17524701)?
The Cytiva HisTrap HP 5 × 1 mL configuration combines Ni Sepharose High Performance resin with convenient prepacked HiTrap columns for His-tagged protein purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
