The Cytiva HiTrap Heparin HP Affinity Column, 1 × 5 mL (17040701) is a prepacked chromatography column containing Heparin Sepharose High Performance for high-resolution purification of biomolecules with an affinity for heparin. The 5 mL column provides a typical loading range of 1 to 15 mg bovine antithrombin III per column and is suitable for purification of coagulation factors, nucleic acid-binding proteins and other heparin-binding biomolecules.
Key Features
- Prepacked with Heparin Sepharose High Performance, providing a ready-to-use affinity medium for high-resolution protein purification
- Single 5 mL column format, designed for preparative laboratory chromatography workflows
- Typical loading range of 1 to 15 mg bovine antithrombin III, providing verified capacity guidance for the 5 mL format
- High-affinity heparin ligand, derived from porcine intestinal mucosa and covalently coupled to the agarose matrix
- Approximately 10 mg heparin per mL of medium, providing the specified ligand substitution level
- Approximately 3 mg bovine antithrombin III binding capacity per mL, supporting preparative affinity separations
- Fine 34 µm spherical particles, supporting high-resolution chromatography and narrow elution peaks
- Recommended flow rate of 5 mL/min, with a maximum flow rate of 20 mL/min for the 5 mL column format
- pH stability from 5 to 10, supporting routine purification and regeneration conditions
- Biocompatible polypropylene column construction, with porous polyethylene frits designed for biomolecule chromatography
- Dual affinity and ion-exchange interaction behaviour, allowing heparin to bind proteins through specific affinity interactions or anionic sulfate groups
- Flexible operation with common chromatography equipment, including syringes, laboratory pumps and ÄKTA chromatography systems
Benefits
- Supports high-resolution purification of antithrombin III, coagulation factors, nucleic acid-binding proteins and other heparin-binding biomolecules.
- Helps minimise downstream concentration requirements by supporting narrow, concentrated elution peaks.
- Reduces column preparation work because the Heparin Sepharose High Performance medium is supplied prepacked and ready to use.
- Provides flexible selectivity because immobilised heparin can function through both affinity and cation-exchange-like interactions.
- Allows convenient method development and scale-up using a standardised HiTrap column format.
Why Choose the Cytiva HiTrap Heparin HP Affinity Column, 1 × 5 mL (17040701)?
The Cytiva HiTrap Heparin HP column combines Heparin Sepharose High Performance with a convenient 5 mL prepacked format for efficient high-resolution purification of heparin-binding proteins. Buy Cytiva HiTrap Heparin HP columns from John Morris Group for affinity chromatography and protein purification workflows in Australia.
