The Cytiva HisTrap FF IMAC Columns, 5 × 1 mL (17531901) are ready-to-use affinity chromatography columns prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilised metal ion affinity chromatography. The pack contains five 1 mL columns with a typical loading range of 1 to 40 mg of His-tagged protein per column.
Key Features
- Prepacked for His-tagged protein purification, using precharged Ni Sepharose 6 Fast Flow affinity medium
- Five 1 mL columns per pack, providing a convenient format for repeated small-scale purification workflows
- Typical loading range of 1 to 40 mg per column, supporting preparative purification of His-tagged proteins
- Approximately 40 mg/mL dynamic binding capacity, providing high protein-binding capability with the Ni Sepharose 6 Fast Flow medium
- Highly cross-linked 6% spherical agarose matrix, providing the base matrix for the affinity medium
- 90 µm average bead size, supporting efficient flow through the prepacked bed
- Recommended flow rate of 1 mL/min, with a maximum flow rate of 4 mL/min for the 1 mL column format
- Broad regular-use pH range from 3 to 12, with short-term stability from pH 2 to 14 after nickel stripping
- Compact 0.7 × 2.5 cm column dimensions, corresponding to the 1 mL HiTrap column format
- 5 bar column hardware pressure limit, equivalent to 0.5 MPa
- Precharged nickel affinity chemistry, with approximately 15 µmol Ni²? capacity per mL of medium
- Flexible chromatography operation, allowing use with a syringe, peristaltic pump or compatible liquid chromatography system
Benefits
- Reduces column preparation time because the affinity medium is supplied prepacked and ready for purification workflows.
- Supports efficient capture of His-tagged proteins through high-capacity nickel affinity chromatography.
- Provides flexibility for different purification conditions through compatibility with commonly used buffers, reducing agents, denaturants and detergents.
- Enables convenient small-scale processing with five separate 1 mL columns in each pack.
- Supports both manual and system-based purification workflows using common laboratory chromatography equipment.
Why Choose the Cytiva HisTrap FF IMAC Columns, 5 × 1 mL (17531901)?
These prepacked columns combine Ni Sepharose 6 Fast Flow with a compact 1 mL bed volume and high binding capacity for efficient preparative purification of His-tagged proteins. Buy Cytiva HisTrap FF columns from John Morris Group for protein purification and affinity chromatography workflows in Australia.
