The Cytiva HisTrap excel IMAC Columns, 5 × 5 mL (17371206) are ready-to-use affinity chromatography columns prepacked with Ni Sepharose excel medium for purification of histidine-tagged proteins. This pack contains five 5 mL columns designed to support direct processing of cell-free, unclarified samples.
Key Features
- Ready-to-use IMAC purification, with each column prepacked with Ni Sepharose excel affinity medium
- Five 5 mL columns per pack, providing a convenient format for repeated His-tagged protein purification workflows
- Direct loading of cell-free, unclarified samples, supported by specialised filters at the top and bottom of the column
- Biocompatible polypropylene construction, with a stopper at the inlet and snap-off outlet end
- 5 mL column dimensions of 1.6 × 2.5 cm, providing the larger HisTrap excel column format
- Flow rates up to 20 mL/min, with 5 mL/min recommended during column wash and elution
- 5 bar hardware pressure limit, equivalent to 0.5 MPa
- At least 10 mg His-tagged protein/mL binding capacity, based on the Ni Sepharose excel medium
- Compatible with common chromatography workflows, including operation with peristaltic pumps and ÄKTA chromatography systems
Benefits
- Reduces sample preparation steps by allowing suitable cell-free, unclarified samples to be loaded directly onto the column.
- Helps minimise loss and degradation of sensitive target proteins by simplifying sample handling before purification.
- Supports efficient purification of secreted histidine-tagged proteins from eukaryotic cell culture supernatants.
- Provides a prepacked and standardised format that avoids the need for manual resin packing before use.
- Offers flexible operation with either peristaltic pumps or compatible chromatography systems.
Why Choose the Cytiva HisTrap excel IMAC Columns, 5 × 5 mL (17371206)?
These prepacked columns combine Ni Sepharose excel medium with a design suited to direct processing of cell-free, unclarified samples, helping streamline His-tagged protein purification. Buy Cytiva HisTrap excel columns from John Morris Group for laboratory protein purification workflows in Australia.
