The Cytiva HisTrap excel IMAC Columns, 5 × 1 mL (17371205), are prepacked affinity chromatography columns for purification of histidine-tagged proteins. Each column has a 1 mL bed volume and is packed with Ni Sepharose excel resin for use in His-tagged protein purification workflows.
Key Features
- Five prepacked 1 mL columns, supplied as a 5 × 1 mL configuration for repeat purification workflows
- Designed for histidine-tagged protein purification, using immobilised metal ion affinity chromatography
- Ni Sepharose excel resin, selected by Cytiva for applications where nickel leakage is an important consideration
- Typical loading range of 1–10 mg per column for His-tagged protein
- Recommended flow rate of 1 mL/min, with a maximum flow rate of 4 mL/min
- Broad regular-use pH range from 2 to 14, supporting varied purification conditions
- 90 µm median particle size for the Ni Sepharose excel resin
- HiTrap 1 mL column format, designed for prepacked chromatography workflows
Benefits
- Reduces column preparation time by providing prepacked affinity columns ready for integration into protein purification workflows.
- Supports repeat experiments and multiple purification runs with five individually sized 1 mL columns in the pack.
- Provides a compact format suited to small-scale capture and purification of histidine-tagged proteins.
- Offers flexibility for method development across a broad range of regular-use pH conditions.
- Provides a Ni Sepharose excel option when nickel leakage is an important consideration during column selection.
Why Choose the Cytiva HisTrap excel IMAC Columns, 5 × 1 mL (17371205)?
The Cytiva HisTrap excel 5 × 1 mL configuration combines prepacked Ni Sepharose excel resin with a compact HiTrap format for convenient histidine-tagged protein purification. Buy from John Morris Group for access to Cytiva chromatography products and technical support for your laboratory workflow.
