The Cytiva His GraviTrap IMAC Columns, 10 × 1 mL (11003399) are prepacked, single-use gravity-flow columns for purification of histidine-tagged proteins by immobilised metal affinity chromatography. Each column contains 1 mL of Ni Sepharose 6 Fast Flow medium and provides an approximate protein binding capacity of 40 mg of histidine-tagged protein per column.
Key Features
- Gravity-flow affinity purification without a chromatography system, providing a straightforward four-stage workflow of equilibration, sample application, washing and elution
- Ni Sepharose 6 Fast Flow medium, using nickel-charged immobilised metal affinity chromatography chemistry for capture of histidine-tagged proteins
- Approximately 40 mg protein binding capacity per column, with actual capacity dependent on the histidine-tagged protein being purified
- 1 mL prepacked bed volume, with ten single-use columns supplied in each pack
- 90 µm average bead size, using highly cross-linked agarose Ni Sepharose 6 Fast Flow medium
- Suitable for clarified and unclarified samples, allowing direct purification of appropriately prepared cell lysates
- Approximately 30-minute purification workflow, depending on sample volume and solution viscosity
- Compatible with native and denaturing conditions, supporting a range of protein purification workflows
- Compatible with common reducing agents, denaturants and detergents, within the concentrations specified by Cytiva
- Short-term pH stability from 2 to 14, specified for Ni2+-stripped medium during a two-hour exposure
- Biocompatible polypropylene column body, with polyethylene frits designed to help prevent the chromatography medium from running dry
Note: Chelating agents such as EDTA, EGTA and citrate should be avoided in buffers because they can interfere with the immobilised nickel ions.
Benefits
- Enables manual purification of histidine-tagged proteins without requiring a dedicated chromatography instrument.
- Reduces preparation time through a prepacked, ready-to-use single-use column format.
- Supports efficient processing of both clarified and suitably prepared unclarified samples, reducing the number of sample-preparation steps required.
- Provides high protein binding capacity in a compact 1 mL format for routine laboratory-scale affinity purification.
- Allows larger sample volumes to be handled by connecting the compatible LabMate reservoir, which Cytiva states can increase one-go loading capacity to approximately 35 mL.
Why Choose the Cytiva His GraviTrap IMAC Columns, 10 × 1 mL (11003399)?
The Cytiva His GraviTrap combines Ni Sepharose 6 Fast Flow chemistry with a convenient gravity-flow format for rapid manual purification of histidine-tagged proteins. Buy from John Morris Group for Cytiva affinity chromatography columns and protein purification solutions in Australia.
