The Cytiva HiPrep 26/10 Desalting Column, 1 × 53 mL (17508701) is a prepacked, ready-to-use chromatography column designed for group separation of high molecular weight substances above Mr 5,000 from low molecular weight substances below Mr 1,000. It is packed with Sephadex G-25 Fine and provides a 53 mL bed volume for laboratory desalting and separation workflows.
Key Features
- Prepacked and ready to use, eliminating the need to manually prepare and pack the chromatography bed
- Sephadex G-25 Fine matrix, using cross-linked dextran with a mean particle size of 90 µm
- 53 mL packed bed volume, with a 100 mm bed height and 26 mm internal column diameter
- Group separation by molecular size, separating high molecular weight substances above Mr 5,000 from low molecular weight substances below Mr 1,000
- Recommended flow range of 9–31 mL/min, corresponding to approximately 100–350 cm/h with water at room temperature
- Maximum flow rate of 40 mL/min, corresponding to approximately 450 cm/h under the stated test conditions
- Broad pH stability from pH 2 to 13, for both long-term and short-term operation
- Polypropylene column construction, with a packed-bed operating pressure limit of 0.15 MPa (1.5 bar)
Note: The recommended sample volume is less than 15 mL. Larger sample volumes can reduce resolution and desalting performance, although multiple HiPrep 26/10 Desalting columns can be connected in series where increased sample capacity is required.
Benefits
- Reduces preparation time by providing a factory-packed column that can be incorporated directly into chromatography workflows.
- Supports efficient separation of salts and other low molecular weight components from larger biomolecules based on molecular size.
- Offers flexible operating conditions through its broad recommended flow-rate range and pH stability.
- Provides predictable sample handling with a defined 53 mL bed volume, 15 mL void volume and recommended sample volume below 15 mL.
- Supports larger sample volumes when required by connecting multiple columns in series while maintaining separation performance.
Why Choose the Cytiva HiPrep 26/10 Desalting Column, 1 × 53 mL (17508701)?
The Cytiva HiPrep 26/10 Desalting Column combines a ready-to-use Sephadex G-25 Fine bed with defined flow, pressure and sample-handling parameters for reproducible group separation. Buy from John Morris Group for Cytiva chromatography columns and protein purification solutions in Australia.
