The Cytiva HiLoad 16/600 Superdex 200 pg SEC Column, 120 mL (28989335) is a prepacked size exclusion chromatography column for rapid preparative separation of proteins, particularly monoclonal antibodies, DNA fragments and other biomolecules. It features a 16 × 600 mm packed bed with 120 mL bed volume and Superdex 200 prep grade resin.
Key Features
- Prepacked for preparative size exclusion chromatography, supporting rapid separation of proteins, monoclonal antibodies, DNA fragments and other biomolecules
- 120 mL packed bed volume, with column dimensions of 16 mm internal diameter × 600 mm bed height
- 34 µm Superdex 200 prep grade resin particles, designed for preparative SEC workflows
- Protein fractionation range of Mr 10,000 to 600,000, covering a broad range of globular proteins
- Dextran fractionation range of Mr 1,000 to 100,000, extending separation capability across different molecular sizes
- Column efficiency greater than 13,000 N/m, supporting high-resolution preparative separations
- Recommended operating flow of 1.0 mL/min, with a recommended maximum operating flow of 1.7 mL/min
- Recommended sample volume up to 5 mL, matched to the HiLoad 16/600 format
- Broad pH stability from pH 3 to 12, supporting a wide range of chromatographic buffer conditions
- Maximum packed-bed pressure of 0.3 MPa (42 psi), defining the operating pressure limit for the column
Benefits
- Supports preparative purification of proteins and other biomolecules without the need for manual column packing.
- Provides a broad molecular-weight fractionation range suited to workflows involving antibodies and other large proteins.
- Offers defined sample-volume and flow-rate parameters for straightforward method setup and reproducible operation.
- Combines a long 600 mm bed with fine resin particles to support efficient size-based separation.
- Provides flexibility across a broad pH range for compatibility with varied purification conditions.
Why Choose the Cytiva HiLoad 16/600 Superdex 200 pg SEC Column, 120 mL (28989335)?
The Cytiva HiLoad 16/600 Superdex 200 pg SEC Column provides a prepacked 120 mL platform for preparative size exclusion chromatography of proteins, monoclonal antibodies, DNA fragments and related biomolecules. Buy from John Morris Group for Cytiva chromatography columns and protein purification solutions in Australia.
