The Cytiva Capto HiRes S 5/50 Cation Exchange Column, 1 mL (29275877) is a prepacked glass column for high-resolution ion exchange chromatography of proteins, peptides, polynucleotides and other biomolecules. Its 5 × 50 mm bed contains strong cation exchange resin with an approximately 9 µm median particle size.
Key Features
- Strong cation exchange chemistry, using sulphopropyl functional groups for biomolecule separation by charge
- High-resolution resin with approximately 9 µm particles, based on the median particle size of the cumulative volume distribution
- Compact 5 × 50 mm packed bed, providing a 1 mL column volume for laboratory-scale purification
- Average loading capacity of approximately 50 mg, with actual capacity dependent on the sample
- Recommended flow range of 0.5 to 2.0 mL/min, providing flexibility when optimising separation conditions
- Maximum packed-bed pressure of 4 MPa, supporting high-resolution chromatography under specified operating conditions
- Broad operational pH range from 2 to 12, with a CIP pH range from 1 to 14
- Operating temperature range from 4 to 40°C, supporting common laboratory chromatography conditions
- Highly cross-linked spherical agarose matrix, providing the base matrix for the Capto HiRes S resin
- Supplied with column connectors and stop plugs, facilitating connection to ÄKTA or other compatible chromatography systems
Benefits
- Perform high-resolution cation exchange separations using a ready-packed laboratory column.
- Purify proteins and other biomolecules according to differences in charge without manually packing resin.
- Optimise resolution and throughput across a recommended 0.5 to 2.0 mL/min flow range.
- Work across varied buffer conditions with a broad operational pH range of 2 to 12.
- Use the compact 1 mL format for laboratory purification and analytical ion exchange workflows.
Why Choose the Cytiva Capto HiRes S 5/50 Cation Exchange Column, 1 mL (29275877)?
The Cytiva Capto HiRes S 5/50 combines a fine-particle strong cation exchange resin with a compact prepacked format for high-resolution biomolecule purification. Buy from John Morris Group for genuine Cytiva chromatography columns and consumables for laboratory ion exchange workflows.
