GSTrap 4B Prepacked Columns, 5 × 5 mL (Part Number 28401748) comprise five 5 mL affinity chromatography columns prepacked with Glutathione Sepharose 4B for purification of GST-tagged proteins. Each column has a 16 × 25 mm packed bed and is designed for protein purification workflows using glutathione affinity chromatography.
Key Features
- Five 5 mL prepacked columns, providing a practical pack format for repeated GST-tagged protein purification runs.
- Glutathione affinity medium, using Glutathione Sepharose 4B with a glutathione ligand coupled to 4% agarose.
- Compact 16 × 25 mm bed dimensions, providing a 5 mL packed-bed column format.
- Average 90 µm particle size, specified for the chromatography medium.
- Defined flow guidance, with recommended sample loading at 0.5 to 2.0 mL/min and washing or elution at 5 mL/min.
- Controlled operating limits, with pH stability from 4 to 13 and a 5 bar hardware pressure limit.
- Flexible operation, suitable for use with a syringe, peristaltic pump, or compatible chromatography system.
- Stored in 20% ethanol, at 4°C to 30°C.
Benefits
- Supports streamlined one-step purification of GST-tagged proteins.
- Allows direct processing of pretreated bacterial lysates and other glutathione-dependent protein samples.
- Uses mild, non-denaturing elution conditions with reduced glutathione to support preservation of protein antigenicity and function.
- Provides a ready-to-use prepacked format for manual, pump-driven, or chromatography system workflows.
Why Choose the GSTrap 4B Prepacked Columns, 5 × 5 mL (Part Number 28401748)?
GSTrap 4B Prepacked Columns provide a convenient 5 mL affinity chromatography format for GST-tagged protein purification. John Morris Group can assist with supplying this five-column pack for suitable laboratory protein purification workflows.
