The Amersham Cy3-dUTP Fluorescent Nucleotide, 25 nmol (PA53022) is 5-amino-propargyl-2'-deoxyuridine 5'-triphosphate coupled to Cy3 fluorescent dye for non-radioactive labelling of DNA probes, oligonucleotides and other nucleic acids. It is supplied as 25 nmol Cy3-dUTP in 10 mM phosphate buffer at pH 7.0 and provides orange Cy3 fluorescence for applications such as microarray detection, FISH and other nucleic acid probe workflows.
Key Features
- Cy3-dUTP fluorescent nucleotide, based on Cy3-AP3-dUTP for incorporation into nucleic acid labelling workflows
- 25 nmol reagent format, supplied as Cy3-dUTP in 10 mM phosphate buffer at pH 7.0
- Orange Cy3 fluorescence, with absorbance maximum at 550 nm and emission maximum at 570 nm
- High sensitivity fluorescent signal, supporting detection where meaningful data from low copy number transcripts is required
- Spectrally resolved dye performance, helping support multicolour analysis within a single sample
- Low nonspecific binding and high photostability, supporting cleaner fluorescent detection workflows
- Water-soluble and pH-insensitive CyDye chemistry, designed for fluorescence analysis in genomic and protein research
- Frozen dark storage, specified at -15°C to -30°C in the dark, with protection from light during handling
Benefits
- Supports non-radioactive labelling of DNA and RNA probes for fluorescence-based analysis.
- Helps enable multicolour workflows in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping and gene mapping.
- Provides Cy3 fluorescence with narrow emission characteristics for clear spectral separation in multiplexed experiments.
- Can be used with many existing rhodamine filter sets, simplifying adoption in suitable fluorescence systems.
- Cold, light-protected handling helps maintain reagent performance across sensitive labelling workflows.
Why Choose the Amersham Cy3-dUTP Fluorescent Nucleotide, 25 nmol (PA53022)?
Choose the Amersham Cy3-dUTP Fluorescent Nucleotide, 25 nmol (PA53022) when your laboratory needs a Cy3-labelled nucleotide for non-radioactive probe labelling and multicolour fluorescence detection. John Morris Group supplies Cytiva and Amersham fluorescent labelling reagents to Australian laboratories, helping you source the correct nucleotide, pack size and supporting documentation for your workflow.
