The Amersham Cy5 Maleimide Mono-Reactive Dye 5-Pack (PA25031) is a Cy5 mono-functional maleimide dye reagent for labelling biological compounds that contain free sulphydryl groups. It is supplied as five foil packs, each containing dried dye to label 1 mg of protein, and is designed for selective thiol labelling in protein, peptide and oligonucleotide workflows.
Key Features
- Cy5 mono-functional maleimide chemistry, ready for labelling compounds containing free sulphydryl groups
- Five premeasured foil packs, each containing dried dye for labelling 1 mg of protein
- Selective thiol labelling route, especially suited to cysteine residues in proteins, peptides and oligonucleotides
- Far-red fluorescent Cy5 signal, suited to detection with CCD cameras, PMTs and some red-sensitive film
- Near-neutral reaction conditions, with maleimide reaction optimised near pH 7.0 and used across pH 7.0 to 7.5
- Defined Cy5 spectral profile, with absorbance maximum at 649 nm and emission maximum at 670 nm
- Specified molar extinction value, listed at 250000 M-1 cm-1 at 650 nm
- Refrigerated dark storage, specified at 2 to 8°C in the dark
Benefits
- Supports selective labelling of thiol-containing biomolecules where broader amine labelling may not be desirable.
- Provides an alternative chemistry when CyDye NHS ester labelling is unsuitable for the target molecule.
- Helps position the dye away from antibody binding sites, supporting retained antibody function after labelling.
- Premeasured foil packs support consistent preparation across five 1 mg protein labelling reactions.
- Far-red Cy5 detection supports imaging or signal capture with suitable CCD, PMT or red-sensitive film systems.
Why Choose the Amersham Cy5 Maleimide Mono-Reactive Dye 5-Pack (PA25031)?
Choose the Amersham Cy5 Maleimide Mono-Reactive Dye 5-Pack (PA25031) when your application needs selective maleimide coupling to free sulphydryl groups with a far-red Cy5 signal. John Morris Group supplies Cytiva and Amersham labelling reagents to Australian laboratories, helping you match the dye chemistry, pack format and documentation to your workflow.
