Amersham Hot Start Mix Ready-To-Go, 0.2 mL, 480 Reactions (28900654) is a preformulated, predispensed, freeze-dried PCR reagent mix supplied in 0.2 mL tubes. The pack provides 480 reactions in a 5 x 96 reaction configuration and contains PuReTaq DNA Polymerase with a Hot Start Activator protein to support specific, reproducible PCR amplification.
Key Features:
- 480-reaction pack configuration, supplied as 0.2 mL tubes in a 5 x 96 reaction format
- Preformulated freeze-dried PCR reagents, combining PuReTaq DNA Polymerase with a Hot Start Activator protein
- Primer-sequestering hot-start chemistry, helping reduce non-specific priming and primer-dimer formation before PCR cycling
- Short initial activation step, requiring 2 minutes before PCR cycling
- Antibody-free formulation, without Taq antibody to avoid mammalian-source contamination associated with antibody-based hot-start systems
- Ready-To-Go reaction setup, requiring water, primers and template DNA to complete the PCR reaction preparation
- 0.2 mL tube format, compatible with most thermal cyclers and arranged in a 96-well configuration
- Ambient-temperature storage, when retained in the supplied pouch with desiccant
- Validated for real-time PCR, supporting applications requiring high specificity and sensitivity
Benefits
- Helps improve amplification specificity by reducing non-specific priming and primer-dimer formation.
- Minimises manual reagent dispensing to support more consistent reaction preparation across larger PCR batches.
- Supports flexible workflow planning through the 0.2 mL, 96-reaction tube configuration.
- Reduces freezer storage requirements through ambient-temperature product storage.
Why Choose the Amersham Hot Start Mix Ready-To-Go, 0.2 mL, 480 Reactions (28900654)?
Amersham Hot Start Mix Ready-To-Go provides a high-volume 480-reaction format with predispensed hot-start PCR reagents for reliable PCR setup. Its primer-sequestering activator system, short activation requirement and ambient-storage format make it suitable for repeatable amplification workflows. John Morris Group can assist with product selection and supply for your PCR applications.
