The Ready-To-Go You-Prime First-Strand Beads, 50 Reactions (27926401) are preformulated, single-dose reaction beads for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA. The kit is supplied in thin-walled 0.5 mL tubes compatible with most thermal cyclers and allows the user to select their preferred first-strand primer.
Key Features
- Preformulated first-strand reaction beads, supplied as single-dose reactions to reduce setup time and pipetting steps
- 50-reaction kit format, packaged in thin-walled 0.5 mL tubes compatible with most thermal cyclers
- No-primer bead formulation, allowing use with oligo(dT), random primers or custom primers complementary to a target mRNA sequence
- Complete reaction chemistry included, with buffer, dATP, dCTP, dGTP, dTTP, murine reverse transcriptase, RNase inhibitor and RNase/DNase-free BSA
- 33 µL final reaction volume, ready for downstream PCR after adding water, Taq DNA polymerase and primers
- Function-tested first-strand performance, confirmed for synthesis of cDNA up to 7.5 kb and RT-PCR from blood samples
- Control mix beads included, with five red-tube control beads containing globin mRNA, buffer and two globin-specific PCR primers
- Ambient-temperature-stable bead format, designed to minimise pipetting errors and reduce cross-contamination risk
Benefits
- Simplifies first-strand cDNA synthesis by supplying predispensed reaction chemistry in single-dose bead format.
- Supports RT-PCR workflows using total RNA or polyadenylated RNA with the user's primer of choice.
- Helps improve consistency across repeated reactions by reducing manual reagent preparation.
- Allows completed first-strand reactions to be used directly in PCR after adding the required PCR components.
- Provides included control beads to help evaluate reaction mix performance and PCR amplification.
Why Choose the Ready-To-Go You-Prime First-Strand Beads, 50 Reactions (27926401)?
Choose the Ready-To-Go You-Prime First-Strand Beads, 50 Reactions (27926401) when you need a convenient RT-PCR reagent format for first-strand cDNA synthesis from total RNA or polyadenylated RNA. John Morris Group supplies Cytiva molecular biology reagents to Australian laboratories with local product support and sourcing assistance.
