The First-Strand cDNA Synthesis Kit, 55 Reactions (27926101) is designed to generate full-length first-strand cDNA from mRNA templates following upstream mRNA extraction, isolation, or purification. After first-strand cDNA synthesis, the sample can be used directly for in vitro amplification by PCR or for second-strand synthesis using the Gubler-Hoffman method. The kit contains sufficient reagents for up to 55 first-strand syntheses, with each synthesis producing enough material for up to 15 amplification reactions.
Key Features
- Full-length first-strand cDNA synthesis, designed for use with mRNA templates after extraction, isolation, or purification
- Supports PCR and second-strand synthesis workflows, allowing the generated sample to be used directly after first-strand synthesis
- Preassembled bulk reaction mixes, helping save time and reduce potential pipetting errors
- Flexible primer selection, with primers supplied separately from the preassembled reaction mixes
- Two included primer options, supplied with pd(N)6 random hexamer primer and NotI-(dT)18 bifunctional primer
- 55 first-strand syntheses per kit, with each synthesis producing enough material for up to 15 amplification reactions
- Complete reagent set, including bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, primers, and instruction booklet
- Cold storage requirement, specified at -15 °C to -30 °C
Benefits
- Supports conversion of mRNA templates into first-strand cDNA for downstream molecular biology workflows.
- Helps streamline PCR preparation by providing the core reagents required in one kit format.
- Gives researchers primer flexibility for different template and library preparation requirements.
- Reduces repetitive reagent setup steps with preassembled reaction mixes.
- Provides a practical 55-reaction format for routine cDNA synthesis work in research laboratories.
Why Choose the First-Strand cDNA Synthesis Kit, 55 Reactions (27926101)?
The First-Strand cDNA Synthesis Kit, 55 Reactions (27926101) provides a complete reagent system for generating full-length first-strand cDNA from mRNA templates, with support for PCR amplification and second-strand synthesis workflows. John Morris Group supplies Cytiva molecular biology and PCR reagents to Australian laboratories that need reliable products for genomics research.
