The Amersham Hot Start Master Mix, 100 Reactions (25150001) is a 2X premixed PCR formulation designed to reduce nonspecific priming and primer-dimer formation during PCR setup. It combines Taq DNA Polymerase, dNTPs, MgCl2, Hot Start Activator protein and stabilisers in a ready-to-use master mix format for 100 reactions.
Key Features
- 2X premixed PCR formulation, reducing the number of reagent additions needed during reaction setup
- Hot Start Activator protein system, designed to sequester primers before PCR and reduce nonspecific priming
- Includes Taq DNA Polymerase and dNTPs, providing key amplification components in a single master mix
- 3 mM MgCl2 concentration in the 2X mix, with additional MgCl2 able to be added for PCR optimisation
- 0.4 mM concentration of each dNTP in the 2X mix, matching the verified Cytiva formulation
- Standard 50 µL reaction setup, using 25 µL of Hot Start Master Mix to reach a 1X final concentration
- 100 reaction pack size, suited to routine PCR method development and amplification workflows
- Storage at -20°C, matching the verified storage requirement for the product
Note: This product is intended for research use only and is not for clinical or in vitro diagnostic procedures.
Benefits
- Helps improve PCR specificity by reducing primer-dimer formation and nonspecific amplification during setup.
- Supports faster reaction preparation by combining multiple PCR components in one master mix.
- Can be used for standard PCR applications, including multiplex PCR and reactions where nonspecific amplification may occur.
- Supports method optimisation through optional MgCl2 supplementation when required.
- Helps reduce experimental variability by simplifying reagent handling and pipetting steps.
Why Choose the Amersham Hot Start Master Mix, 100 Reactions (25150001)?
The Amersham Hot Start Master Mix, 100 Reactions (25150001) provides a verified 2X hot start PCR formulation with Taq DNA Polymerase, Hot Start Activator protein and dNTPs for routine amplification workflows. John Morris Group can support Australian laboratories with Amersham PCR reagents and related molecular biology consumables.
