TempliPhi 2000 Reaction Kit, 2000 Reactions (28964286) is a rolling circle amplification reaction kit for high-throughput preparation of circular DNA templates. It is supplied for 2000 reactions and includes TempliPhi premix, denature buffer, enzyme mix, and positive control pUC19 DNA.
Key Features
- High-throughput 2000 reaction format, suited to laboratories processing larger numbers of circular DNA templates
- Rolling circle amplification workflow, designed to prepare DNA sequencing templates using isothermal RCA
- Included premix and reagents, with TempliPhi premix, denature buffer, enzyme mix, and positive control pUC19 DNA
- Positive control DNA supplied, using pUC19 DNA at 2 ng/µL
- Circular DNA template application, for preparation of templates for cycle sequencing, cloning, and transformation
- Sample input flexibility, supporting bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular DNA
- 16 to 18 h test time, suitable for overnight amplification workflows
- Frozen storage requirements, with TempliPhi premix stored at -70°C and handled cold during thawing
Benefits
- Supports high-throughput preparation of circular DNA templates for sequencing and molecular biology workflows.
- Reduces the need for separate template preparation steps by allowing amplified DNA to be used directly for cycle sequencing.
- Helps streamline larger batch workflows with a 2000 reaction pack size.
- Included control DNA supports routine workflow checks during amplification setup.
Why Choose the TempliPhi 2000 Reaction Kit, 2000 Reactions (28964286)?
The TempliPhi 2000 Reaction Kit, 2000 Reactions (28964286) is suited to laboratories preparing circular DNA templates at higher throughput using an RCA-based workflow. John Morris Group can assist Australian laboratories with Cytiva TempliPhi kits and related molecular biology consumables.
