TempliPhi Sequence Resolver Kit, 20 Reactions (28903529), is designed to amplify difficult circular DNA templates for successful DNA sequencing. The kit uses highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides in isothermal rolling circle amplification.
Key Features:
- Designed for difficult DNA templates, supporting sequencing of templates with high GC content and secondary structures
- Modified-nucleotide rolling circle amplification, using Phi29 DNA polymerase to amplify circular templates before sequencing
- Addresses common sequence-finishing issues, including repeats, sequencing stops and sequence compressions
- Suitable for small and large circular templates, including plasmids, M13, fosmids and bacterial artificial chromosomes
- Flexible starting material options, supporting purified DNA, glycerol stock, liquid culture or colonies
- Direct sequencing workflow, with amplified DNA suitable for direct use in cycle sequencing reactions without purification
- Defined 20-reaction kit format, supplied with Sample Buffer, Reaction Buffer and Enzyme Mix
- Overnight protocol, requiring less than 20 minutes of hands-on preparation and an 18-hour reaction time
Benefits
- Helps improve sequencing success when conventional finishing approaches struggle with difficult templates.
- Eliminates the need to use dGTP chemistry and sequencing additives for the stated difficult-template workflow.
- Reduces preparation work by allowing templates to be prepared directly from multiple starting material types.
- Supports a streamlined path from amplification to cycle sequencing without a separate purification step.
Why Choose the TempliPhi Sequence Resolver Kit, 20 Reactions (28903529)?
TempliPhi Sequence Resolver Kit, 20 Reactions (28903529), provides a practical amplification workflow for circular DNA templates that are difficult to sequence because of sequence complexity or secondary structure. Its modified-nucleotide rolling circle amplification chemistry supports direct use of amplified DNA in cycle sequencing reactions. John Morris Group can assist with product selection and ordering for part 28903529.
