The TempliPhi Large Construct Kit, 1000 Reactions (25640080) is an isothermal rolling circle amplification kit for preparing DNA sequencing templates from large DNA vectors such as fosmids and BACs. The kit uses Phi29 DNA polymerase to amplify circular DNA templates and is supplied for 1000 reactions.
Key Features
- Designed for large DNA constructs, specifically supporting BAC and fosmid DNA sequencing template preparation
- 1000 reaction kit format, matching the verified TempliPhi Large Construct Kit variant
- Isothermal rolling circle amplification, enabling circular DNA template amplification without thermal cycling
- Phi29 DNA polymerase-based method, supporting high fidelity DNA replication through proofreading activity
- 18 h amplification at 30 °C, providing overnight amplification under isothermal conditions
- Generates 5 µg template DNA, allowing amplified DNA to be used directly in cycle sequencing reactions without purification
- Flexible starting material options, including purified templates, bacterial culture, glycerol stocks, colonies, or circular DNA samples
- Supplied with sample buffer, reaction buffer, and enzyme mix, supporting the required amplification workflow
Benefits
- Helps streamline sequencing template preparation for large constructs where conventional plasmid-style preparation can be time-consuming.
- Supports high-throughput workflows with a simplified protocol and reduced hands-on time.
- Eliminates the need for thermal cycling during amplification, simplifying setup for routine sequencing template preparation.
- Allows amplified double-stranded DNA products to be sequenced with forward and reverse primers.
- Reduces reorder uncertainty by clearly matching part 25640080 with the 1000 reaction Large Construct Kit format.
Why Choose the TempliPhi Large Construct Kit, 1000 Reactions (25640080)?
Choose the TempliPhi Large Construct Kit, 1000 Reactions (25640080) when you need the verified large-construct RCA kit for BAC or fosmid DNA sequencing template preparation. John Morris Group can help confirm suitability for your molecular biology, sequencing, or high-throughput sample preparation workflow.
