The TempliPhi 500 Amplification Kit, 500 Reactions (25640050) is a rolling circle amplification kit for preparing DNA sequencing templates from circular DNA. Based on Phi29 DNA polymerase, it amplifies single-stranded or double-stranded circular DNA using an isothermal RCA method.
Key Features
- 500 reaction kit format, containing sufficient reagents for 500 sequencing template amplification reactions
- Phi29 DNA polymerase RCA chemistry, used to exponentially amplify circular DNA templates
- Isothermal 30°C amplification, supporting amplification without thermal cycling
- Single-stranded and double-stranded circular DNA support, suitable for circular template amplification workflows
- Microgram DNA yield from low input amounts, designed to generate microgram quantities from picogram amounts of starting material
- Direct sequencing-template use, allowing amplified DNA to be used directly in cycle sequencing reactions without purification
- Separate kit components, with sample buffer, reaction buffer and enzyme mix supplied separately for the 100 and 500 reaction kits
- Cold storage requirement, with long-term storage at -70°C and dry ice shipping listed by the matched source
Note: The product booklet states these products are intended for research use only and not for clinical or in vitro diagnostic procedures.
Benefits
- Helps prepare circular DNA templates for cycle sequencing, cloning and transformation workflows.
- Reduces the need for conventional plasmid or M13 DNA purification in suitable workflows.
- Supports amplification from bacterial or M13 cultures, colonies, plaques, glycerol stocks or purified circular DNA.
- Provides a larger 500 reaction format for laboratories running repeated sequencing-template preparation workflows.
Why Choose the TempliPhi 500 Amplification Kit, 500 Reactions (25640050)?
The TempliPhi 500 Amplification Kit, 500 Reactions (25640050) provides a verified Phi29 DNA polymerase RCA format for circular DNA template preparation. John Morris Group can help Australian laboratories source the correct TempliPhi 500 reaction kit for sequencing-template amplification workflows.
