The TempliPhi 100 Amplification Kit, 100 Reactions (25640010) is an amplification kit for preparing DNA sequencing templates from circular DNA. Based on Phi29 DNA polymerase rolling circle amplification, it enables isothermal amplification of single- or double-stranded circular DNA without thermal cycling.
Key Features
- 100 reaction kit format, matching the specified TempliPhi 100 pack size
- Phi29 DNA polymerase RCA chemistry, supporting rolling circle amplification of circular DNA templates
- Isothermal 30 °C amplification, enabling amplification without thermal cycling
- 4 to 18 h test time, depending on the starting material used
- Microgram DNA output from picogram input, helping prepare sequencing template quantities from low starting material
- Direct sequencing workflow support, allowing amplified DNA to be used directly for cycle sequencing without purification
- Multiple circular DNA sample inputs, including bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, and purified plasmid or M13 DNA
- Separate unmixed kit components, supplied for enhanced stability in the 100 reaction format
Benefits
- Helps prepare circular DNA templates for sequencing, cloning, and transformation workflows.
- Reduces hands-on preparation time compared with traditional plasmid template preparation methods.
- Supports uniform DNA output, helping reduce the need for prior quantification before sequencing reactions.
- Clear 100 reaction identification helps avoid selecting the 500 reaction TempliPhi kit variant.
Why Choose the TempliPhi 100 Amplification Kit, 100 Reactions (25640010)?
The TempliPhi 100 Amplification Kit, 100 Reactions (25640010) provides a defined Phi29 rolling circle amplification workflow for circular DNA template preparation. John Morris Group supplies molecular biology reagents and amplification kits across Australia, helping laboratories source the correct TempliPhi reaction size for their sequencing workflow.
