The ExoProStar PCR Clean-Up Kit, 5000 Reactions (US78225) is an enzymatic PCR and sequencing reaction clean-up kit for removing unincorporated primers and dNTPs from completed PCR reactions. It combines Exonuclease I and Alkaline Phosphatase in a two-tube format for flexible reaction preparation before sequencing or other downstream workflows.
Key Features
- Enzymatic PCR clean-up, designed to remove unincorporated primers and dNTPs from PCR reactions prior to sequencing
- Two-enzyme system, using Exonuclease I and Alkaline Phosphatase for clean-up of amplification reactions
- Separate tube format, with the enzymes provided in two separate tubes for flexible reaction setup
- 5000 reaction pack, supplied for laboratories running high-throughput PCR clean-up workflows
- 30 minute protocol, with a 15 minute digestion step followed by 15 minutes of heat inactivation
- Scalable reaction setup, allowing enzyme additions to be adjusted in proportion to the PCR reaction volume
- Cold storage requirement, with storage specified at -20°C for the reagent kit
Benefits
- Reduces manual purification steps before DNA sequencing or other downstream molecular workflows.
- Helps keep the target PCR product intact while removing residual primers and nucleotides.
- Supports both manual and automated processing where an enzymatic clean-up workflow is preferred.
- The two-tube format gives users control over enzyme addition during reaction preparation.
Why Choose the ExoProStar PCR Clean-Up Kit, 5000 Reactions (US78225)?
The ExoProStar PCR Clean-Up Kit, 5000 Reactions (US78225) is a practical choice for enzymatic clean-up of PCR and sequencing setup reactions where primer and dNTP removal is required. John Morris Group supplies ExoProStar reagents to Australian laboratories with support for product selection and ordering.
