The ExoProStar 1-Step PCR Clean-Up, 5000 Reactions (US77750) is an enzymatic PCR and sequence reaction clean-up reagent for removing unincorporated primers and dNTPs before downstream analysis. This 5000 reaction format combines Exonuclease I and Alkaline Phosphatase enzymes in one stable mix for a simplified one-step clean-up workflow.
Key Features
- Ready-mixed enzyme formulation, combining Exonuclease I and Alkaline Phosphatase in one tube
- Removes residual primers and dNTPs, preparing PCR and sequence reaction products for downstream analysis
- One pipetting step, helping simplify reaction set-up compared with multi-component clean-up workflows
- Fast 30-minute protocol, with 15 minutes at 37°C followed by 15 minutes at 80°C for enzyme inactivation
- 100% PCR product recovery, supporting clean-up while keeping the target PCR product intact
- Scalable reaction format, allowing reagent volume to be adjusted in proportion to the PCR reaction mix
- Automation-friendly workflow, supporting use in suitable manual or automated laboratory processes
- 5000 reaction pack, suited to laboratories processing larger numbers of PCR clean-up reactions
Benefits
- Reduces clean-up handling by combining two enzymatic activities in a single stable mix.
- Helps prepare PCR products for sequencing or other downstream molecular biology workflows.
- Supports efficient routine use in laboratories with high-volume PCR clean-up requirements.
- Minimises set-up complexity with a single-tube, one-step reagent format.
- Can be integrated into suitable manual or automated clean-up workflows.
Why Choose the ExoProStar 1-Step PCR Clean-Up, 5000 Reactions (US77750)?
Choose this ExoProStar 1-Step reagent when you need a large-format enzymatic PCR clean-up option for removing primers and dNTPs before sequencing or downstream analysis. John Morris Group supplies ExoProStar molecular biology reagents in Australia with local product support and ordering assistance.
