The Tissue and Cells genomicPrep Mini Spin Kit, 250 Preps (28904276) is designed for rapid extraction and purification of high molecular weight genomic DNA from animal tissues and cultured mammalian cells. The kit uses DNA spin columns with a silica membrane workflow and supports tissue preparation in approximately 90 minutes or cultured-cell preparation in approximately 45 minutes.
Key Features:
- Animal tissue and cultured-cell workflows, suitable for 5 to 50 mg of animal tissue or up to 5 × 10⁶ cultured mammalian cells
- High molecular weight genomic DNA, produces purified DNA with a product size greater than 20 kb
- Silica-membrane purification, uses Proteinase K lysis, chaotropic binding conditions, washing, and low ionic-strength elution
- Consistent purification capacity, with a binding capacity greater than 35 µg of genomic DNA per preparation
- Defined tissue and cell yields, typically provides 0.5 to 1.5 µg DNA per mg of tissue or 10 to 20 µg genomic DNA from 5 × 10⁶ cultured cells
- High-purity DNA output, with an A260/A280 ratio greater than 1.75 for tissue and cultured-cell preparations
- Optional RNA removal, allows RNase A treatment to be included where RNA-free genomic DNA is required
- 250-preparation format, supplies reagents and columns for 250 genomic DNA purifications
Benefits:
- Prepare purified genomic DNA from common animal tissue and mammalian cell samples using one defined extraction system.
- Support downstream cloning, restriction enzyme digestion, PCR amplification, genotyping, and real-time PCR workflows.
- Process tissue and cell samples with separate preparation times suited to their respective workflows.
- Use a 250-preparation kit format for routine laboratory genomic DNA extraction requirements.
Why Choose the Tissue and Cells genomicPrep Mini Spin Kit, 250 Preps (28904276)?
The Tissue and Cells genomicPrep Mini Spin Kit, 250 Preps (28904276) provides a silica-membrane method for extracting high molecular weight genomic DNA from animal tissues and cultured mammalian cells. Its defined input ranges, purification capacity, and 250-preparation format support routine molecular biology workflows. John Morris Group can assist with ordering and product selection.
