The PlasmidPrep Mini Spin Kit, 250 Purifications (28904270) is designed for rapid purification of plasmid DNA from transformed E. coli cultures. It uses modified alkaline lysis and a silica-membrane spin-column workflow to isolate high-quality plasmid DNA from 1.5 mL culture volumes in approximately 9 minutes.
Key Features:
- Rapid plasmid DNA purification, with an approximately 9-minute protocol for routine mini-preparation workflows
- Modified alkaline lysis workflow, supports efficient release of plasmid DNA from transformed E. coli cells
- Silica-membrane purification, uses chaotropic salts to promote selective plasmid DNA binding while contaminants are removed during washing
- Defined culture-volume format, processes 1.5 mL culture volumes for the 250-purification kit variant
- Typical plasmid DNA yield of 6 to 15 µg, supports routine downstream molecular biology workflows
- Purity value of 1.7, as listed for this 250-preparation variant
- No organic solvent requirement, avoids phenol and chloroform-based purification methods
- Complete 250-purification kit, supplied with silica-membrane spin columns, buffers, collection tubes, and protocol materials
Benefits:
- Prepare plasmid DNA for restriction enzyme analysis, ligation, cloning, DNA sequencing, PCR, transformation, and labelled probe generation.
- Process routine plasmid mini-preparations with a defined spin-column workflow and minimal handling steps.
- Support laboratory throughput with reagents and consumables for 250 plasmid DNA purifications.
- Use colour-coded tubes and columns to assist with clear protocol navigation during the extraction process.
Why Choose the PlasmidPrep Mini Spin Kit, 250 Purifications (28904270)?
The PlasmidPrep Mini Spin Kit, 250 Purifications (28904270) provides a practical plasmid DNA extraction method for laboratories processing transformed E. coli cultures. Its rapid alkaline lysis and silica-membrane workflow supports high-quality plasmid DNA preparation for common downstream molecular biology applications. John Morris Group can assist with ordering and product selection.
