The Bacteria genomicPrep Mini Spin Kit, 250 Preps (28904259) is designed for the rapid extraction and purification of high molecular weight genomic DNA from Gram-negative and Gram-positive bacteria. This 250-preparation kit uses a silica membrane mini spin-column workflow to produce intact gDNA larger than 20 kbp for molecular biology applications.
Key Features:
- Dedicated bacterial gDNA extraction workflow, with separate protocols for Gram-negative and Gram-positive bacteria
- Silica membrane purification, uses lysis solutions and Proteinase K before gDNA binding, washing, and elution
- High molecular weight DNA recovery, produces gDNA larger than 20 kbp with protocols designed to minimise shearing
- Defined bacterial input range, supports 1 to 4 × 10⁹ bacterial cells per purification
- Typical bacterial gDNA yields, delivers 4 to 12 µg from Gram-negative bacteria and 5 to 10 µg from Gram-positive bacteria, based on 2 × 10⁹ cells
- Efficient processing times, provides approximately 40-minute processing for Gram-negative bacteria and approximately 60-minute processing for Gram-positive bacteria
- Optional RNA removal step, enables preparation of RNA-free genomic DNA when included in the workflow
- 250-preparation format, provides reagents and columns for 250 bacterial genomic DNA purifications
Benefits:
- Prepare purified bacterial gDNA for molecular biology workflows including cloning, PCR, genotyping, and restriction enzyme digestion.
- Use dedicated Gram-negative and Gram-positive protocols to support different bacterial cell-wall structures.
- Generate intact, high molecular weight gDNA suitable for downstream real-time PCR and long PCR applications.
- Support routine laboratory throughput with a 250-preparation kit format.
Why Choose the Bacteria genomicPrep Mini Spin Kit, 250 Preps (28904259)?
The Bacteria genomicPrep Mini Spin Kit, 250 Preps (28904259) provides a defined workflow for bacterial genomic DNA purification from both Gram-negative and Gram-positive organisms. Its silica membrane extraction method combines high molecular weight DNA recovery with practical processing times for routine molecular biology applications. John Morris Group can assist with ordering and product selection.
