The AutoSeq G-50 Columns, 1000 Columns (27534003) are prepacked spin columns for removing unincorporated fluorescent dye-terminators from cycle sequencing reactions before automated sequencing. These columns are packed with Sephadex G-50 DNA Grade F and supplied in a 1000 column pack for high-throughput sequencing clean-up workflows.
Key Features
- Designed for dye-terminator removal, supporting clean-up of cycle sequencing reactions before automated sequencing
- Prepacked Sephadex G-50 DNA Grade F columns, ready for spin-column purification workflows
- 12 to 25 µL sample volume range, allowing sequencing reaction application without dilution
- Purification in less than 4 min, from sample application through to collection of purified product
- 1000 column pack size, suitable for laboratories processing larger numbers of sequencing reactions
- Pre-equilibrated column format, supplied in double-distilled water with 0.05% Kathon CG/ICP Biocide preservative
- 50 to 150 µm dry particle size, confirmed for the Sephadex G-50 DNA Grade F media
Benefits
- Helps remove residual fluorescent dye-terminators that can interfere with automated sequencing analysis.
- Supports faster sequencing reaction clean-up compared with longer precipitation-based workflows.
- Reduces preparation steps by using ready-to-use, prepacked columns.
- Provides a bulk 1000 column format for facilities with repeat or high-throughput sequencing clean-up needs.
Why Choose the AutoSeq G-50 Columns, 1000 Columns (27534003)?
The AutoSeq G-50 Columns, 1000 Columns (27534003) are a practical option for laboratories that need a verified Cytiva AutoSeq G-50 bulk pack for dye-terminator removal before automated sequencing. John Morris Group supplies molecular biology clean-up products across Australia with local support for laboratory procurement and product selection.
