The AutoSeq G-50 Columns, Pack of 250 (27534002) are ready-to-use spin columns for DNA sequencing cleanup. These columns are designed to remove unincorporated fluorescent dye-terminators from cycle sequencing reactions before analysis on automated sequencers. Each column is prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide as a preservative.
Key Features
- Designed for dye-terminator removal, helping clean up cycle sequencing reactions before automated DNA sequencing
- Prepacked Sephadex G-50 DNA Grade F columns, providing a ready-to-use gel filtration format for sequencing cleanup
- 50-150 µm dry particle size, matching the verified Sephadex G-50 resin specification for this product
- Pre-equilibrated in double-distilled water, avoiding traditional salt-containing buffers that may cause electrophoretic artefacts on salt-sensitive automated sequencers
- 0.05% Kathon CG/ICP Biocide preservative, supporting storage of the pre-equilibrated column format
- Fast sample-to-collection workflow, enabling sample application through to collection of purified product in less than 4 min
- Pack of 250 columns, suitable for laboratories processing repeated sequencing cleanup workflows
Benefits
- Helps improve automated sequencing results by reducing residual dye-terminators that can obscure data.
- Reduces hands-on preparation by using prepacked, pre-equilibrated columns.
- Supports faster turnaround for routine cycle sequencing cleanup workflows.
- Provides a convenient bulk pack for laboratories with ongoing sequencing preparation needs.
Why Choose the AutoSeq G-50 Columns, Pack of 250 (27534002)?
The AutoSeq G-50 Columns, Pack of 250 (27534002) provide a convenient Sephadex G-50 DNA Grade F cleanup format for removing fluorescent dye-terminators from cycle sequencing reactions. They are suited to Australian molecular biology laboratories preparing samples for automated DNA sequencing. John Morris Group can help confirm availability and suitability for your sequencing cleanup workflow.
