The Amersham MicroSpin G-50 Columns, 250 Columns (27533002) are prepacked spin columns for rapid DNA purification using typical microfuge equipment. They are designed for desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions, and removal of labelled nucleotides from DNA labelling reactions.
Key Features
- Prepacked Sephadex G-50 DNA Grade columns, supplied equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide
- 250-column pack format, matching the verified 27533002 variant for 250 preps
- Optimised for 12 to 50 µL sample volumes, supporting good product yield and purity
- Rapid labelled DNA purification, suitable for labelled DNA greater than 20 bases
- 25 to 50 µL labelled DNA clean-up range, supporting removal of unincorporated labelled nucleotides by spin-column chromatography
- Buffer exchange and desalting capability, suitable for enzymatic reactions of 10 to 100 µL after heat denaturation and phenol extraction
- Enzyme-compatible clean-up, designed not to remove or denature enzyme
- Room-temperature storage, specified at 20 to 25 °C
Benefits
- Helps streamline DNA clean-up using standard microfuge equipment.
- Supports routine clean-up after cycle sequencing and DNA labelling reactions.
- Provides convenient prepacked columns to reduce manual preparation time.
- Helps maintain sample integrity in workflows where enzyme retention is important.
- Pack of 250 supports higher-throughput or frequently repeated molecular biology workflows.
Why Choose the Amersham MicroSpin G-50 Columns, 250 Columns (27533002)?
Choose the Amersham MicroSpin G-50 Columns, 250 Columns (27533002) when you need a verified 250-column format for DNA clean-up, desalting, buffer exchange, and removal of small molecules from labelled DNA reactions. John Morris Group supplies Amersham and Cytiva molecular biology products for Australian laboratories, with support to confirm suitability and availability for your workflow.
