The Amersham MicroSpin G-50 Columns, 50 Columns (27533001) are prepacked spin columns for rapid DNA purification using standard microfuge equipment. The columns are designed for desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions, and removal of labelled nucleotides from DNA labelling reactions. They are prepacked with Sephadex G-50 DNA Grade equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
Key Features
- Rapid DNA purification spin-column format, designed for use with typical microfuge equipment
- Sephadex G-50 DNA Grade matrix, prepacked for convenient gel filtration purification
- Pre-equilibrated column chemistry, supplied in TE buffer with 0.05% Kathon CG/ICP Biocide
- Suitable for labelled DNA >20 bases, supporting removal of unincorporated labelled nucleotides from smaller molecules in solution
- 25 to 50 µL labelled DNA purification volume, for spin-column chromatography applications
- 10 to 100 µL buffer exchange and desalting volume, following heat denaturation and phenol extraction of enzymatic reactions
- Does not remove or denature enzyme, supporting compatible downstream enzymatic workflows
- 50 purification pack, supplied with collection tubes for routine laboratory use
Benefits
- Helps streamline cleanup of DNA labelling and cycle sequencing reaction products.
- Supports rapid desalting and buffer exchange without complex setup or gravity-flow columns.
- Provides a convenient prepacked format that reduces column preparation time.
- Fits molecular biology workflows requiring purification of labelled DNA from smaller contaminants.
Why Choose the Amersham MicroSpin G-50 Columns, 50 Columns (27533001)?
The Amersham MicroSpin G-50 Columns (27533001) provide a ready-to-use spin-column option for DNA desalting, buffer exchange, dye terminator removal, and labelled nucleotide cleanup. With Sephadex G-50 DNA Grade media and a 50-column pack size, they are suited to recurring molecular biology purification workflows. John Morris Group supplies Amersham and Cytiva purification products for Australian laboratories.
