The Amersham MicroSpin G-25 Columns, Pack of 50 (27532501) are prepacked spin columns for rapid DNA purification by gel filtration. Each column contains Sephadex G-25 DNA Grade F resin and is designed for desalting, buffer exchange and removal of unincorporated nucleotides from end-labelled oligonucleotides.
Key Features
- Prepacked G-25 spin-column format, supplied ready for DNA purification by gel filtration
- Sephadex G-25 DNA Grade F resin, providing the packed resin bed used for desalting and buffer exchange
- 10 to 100 µL working volume, suitable for PCR products and other DNA samples processed by spin-column chromatography
- 5000 Da MWCO pore size, supporting separation of DNA from smaller buffer salts and unincorporated nucleotides
- 20 to 80 µm dry particle size, defining the resin particle-size range used in the column
- Ready-to-use preparation, pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide
- Fast processing workflow, requiring less than 4 minutes from sample application to collection of purified product
- Tested for nuclease activity, including nickase, single-stranded exonuclease, double-stranded exonuclease and RNase assays
Benefits
- Speeds up DNA desalting and buffer exchange without requiring manual column packing.
- Helps clean up PCR products, oligonucleotides and small DNA fragments following synthesis or labelling reactions.
- Supports removal of unincorporated nucleotides from end-labelled oligonucleotides of at least 10 bases in length.
- Maintains enzymes in the sample workflow because the columns are not intended to remove or denature enzyme.
- Pack of 50 supports routine molecular biology workflows and repeat clean-up runs.
Why Choose the Amersham MicroSpin G-25 Columns, Pack of 50 (27532501)?
Choose the Amersham MicroSpin G-25 Columns, Pack of 50 (27532501) when you need fast, prepacked G-25 columns for DNA desalting, buffer exchange and oligonucleotide purification. John Morris Group supplies Cytiva and Amersham molecular biology consumables to Australian laboratories with local product support and sourcing assistance.
