The Amersham MicroSpin S-400 HR Columns, 50 Pack (27514001) are Cytiva spin columns for rapid purification of PCR products greater than 200 bp from unincorporated primers less than 32-mers and nucleotides. They use spin-column chromatography and are prepacked with Sephacryl S-400 HR pre-equilibrated in TE buffer. This 50-column pack supports post-PCR clean-up prior to cloning or a second amplification reaction, as well as other DNA clean-up applications.
Key Features
- Purifies PCR products greater than 200 bp, removing unincorporated primers less than 32-mers and nucleotides
- Spin-column chromatography format, supporting rapid DNA clean-up in small sample volumes
- Flexible sample volume handling, accommodating 25 to 50 µL for post-PCR clean-up or 25 to 100 µL for other applications
- Prepacked Sephacryl S-400 HR medium, supplied pre-equilibrated in TE buffer
- Ready-to-use column format, requiring less than 4 minutes from sample application to purified product collection
- Nuclease tested components, tested in nickase, single-stranded exonuclease, double-stranded exonuclease, and RNase assays
- Pack of 50 columns, suitable for routine PCR clean-up and primer removal workflows
Benefits
- Streamlines clean-up after PCR by removing unwanted primers and nucleotides before downstream work.
- Supports preparation of PCR products before cloning, second amplification reactions, or Sanger sequencing workflows.
- Reduces handling time with a ready-to-use spin-column format.
- Helps maintain workflow consistency for small-volume DNA purification.
- Provides a convenient 50-column pack for laboratories performing repeated PCR clean-up tasks.
Why Choose the Amersham MicroSpin S-400 HR Columns, 50 Pack (27514001)?
The Amersham MicroSpin S-400 HR Columns, 50 Pack (27514001) provide a rapid, ready-to-use format for PCR product clean-up, primer removal, and nucleotide removal using Sephacryl S-400 HR spin-column chromatography. John Morris Group supplies Cytiva and Amersham molecular biology consumables to Australian laboratories that need reliable products for DNA purification and genomics workflows.
