The Amersham MicroSpin S-300 HR Columns, 50 Pack (27513001) are spin columns for rapid purification of PCR products from unincorporated primers and nucleotides. These columns are prepacked with Sephacryl S-300 HR media pre-equilibrated in TE buffer and are used for post-PCR clean-up, desalting, buffer exchange and primer removal workflows.
Key Features
- Designed for PCR product purification, removing unincorporated primers under 20-mers and nucleotides from PCR products greater than 100 bp
- 25 to 50 µL post-PCR clean-up volume, supporting clean-up prior to sequencing
- Spin-column chromatography format, providing a compact workflow for molecular biology sample clean-up
- Prepacked Sephacryl S-300 HR media, supplied pre-equilibrated in TE buffer for convenient use
- Rapid processing workflow, requiring less than 4 minutes from sample application to collection of purified product
- Greater than 80% sample recovery, as listed by the verified reseller source
- Tested nuclease performance, with testing in nickase, single-stranded exonuclease, double-stranded exonuclease and RNase assays
- 50-column pack configuration, matching the selected 27513001 product variant
Benefits
- Helps prepare PCR products for sequencing by removing excess primers and nucleotides.
- Supports desalting and buffer exchange workflows for nucleic acid purification.
- Provides a ready-to-use column format that reduces preparation steps.
- Useful for purification of alkaline-denatured plasmid DNA prior to sequencing.
Why Choose the Amersham MicroSpin S-300 HR Columns, 50 Pack (27513001)?
The Amersham MicroSpin S-300 HR Columns, 50 Pack (27513001) provide a convenient spin-column format for PCR clean-up, primer removal and DNA purification workflows. John Morris Group supplies Cytiva and Amersham molecular biology consumables across Australia with local support for product selection and ordering.
