NICK DNA Purification Columns, 50 Columns (17085502) are disposable gravity flow columns for purification of nick-translated DNA fragments and labelled probes. The columns are prepacked with Sephadex G-50 DNA Grade resin and are designed to separate labelled DNA from unincorporated labelled nucleotides after DNA labelling reactions.
Key Features
- Gravity flow purification, allowing DNA clean-up without pumps or centrifugation
- Prepacked Sephadex G-50 DNA Grade matrix, supplied ready for gel filtration workflows
- Suitable for labelled DNA greater than 20 bp, with no maximum oligonucleotide length stated for purification
- 1 to 100 µL input sample volume, matching small-volume DNA labelling reaction clean-up
- High DNA recovery, with greater than 90% yield or recovery of DNA
- Low salt carryover, with recovered DNA typically showing less than 0.2% salt contamination
- Pack of 50 columns, supplied for 50 purifications
Benefits
- Supports rapid purification of nick-translated DNA fragments after labelling reactions.
- Helps separate labelled probes from unincorporated labelled nucleotides before downstream work.
- Provides a simple gravity-based workflow for hybridisation applications.
- Uses single-use prepacked columns to reduce setup steps and simplify routine DNA clean-up.
Why Choose the NICK DNA Purification Columns, 50 Columns (17085502)?
NICK DNA Purification Columns provide a practical 50-column format for gravity flow purification of labelled DNA using Sephadex G-50 DNA Grade gel filtration. John Morris Group supplies Cytiva and Amersham molecular biology consumables to Australian laboratories with local product support and ordering assistance.
