NAP Amersham NAP-10 DNA Purification Columns, 20 Pack (17085401) are disposable gravity-flow columns for purification of oligonucleotides and small DNA fragments. Each column is prepacked with Sephadex G-25 DNA Grade resin for desalting, buffer exchange and removal of unincorporated nucleotides from end-labelled oligonucleotides.
Key Features
- Gravity-flow DNA purification, requiring no positive pressure during column operation
- Prepacked Sephadex G-25 DNA Grade matrix, supplied ready for gel filtration cleanup workflows
- NAP-10 format for up to 1 mL samples, suited to small-scale nucleic acid purification
- High DNA recovery, with reported yield/recovery greater than 90%
- Low residual salt level, with recovered DNA typically containing less than 3% salt contamination
- Suitable for DNA greater than 10 bases, including oligonucleotides and very small DNA fragments
- 20-column pack, matching the 17085401 pack configuration for routine laboratory use
Benefits
- Helps prepare oligonucleotides and small DNA fragments for downstream PCR, sequencing and labelling workflows.
- Supports desalting and buffer exchange without centrifugation or pressure-based purification steps.
- Provides a convenient disposable column format for reducing carryover from salts, buffers and unincorporated nucleotides.
- Allows laboratories to select a nuclease-free buffer such as water or TE for equilibration and elution.
Why Choose the NAP Amersham NAP-10 DNA Purification Columns, 20 Pack (17085401)?
These NAP-10 columns offer a simple gravity-flow format for small-volume DNA and oligonucleotide cleanup using Sephadex G-25 DNA Grade resin. John Morris Group supplies Cytiva and Amersham nucleic acid purification products for Australian laboratories requiring verified consumables for molecular biology workflows.
