The Cytiva Protein Select HiTrap Column, 5 x 5 mL (17542154) is a pack of prepacked affinity chromatography columns for purifying recombinant proteins using the self-cleaving Cytiva Protein Select tag. The column format supports one-step affinity purification, tag cleavage, and tag removal, helping produce tag-free protein without protease treatment.
Key Features
- Prepacked HiTrap column format, supplied as 5 x 5 mL columns for recombinant protein purification workflows
- Self-cleaving tag technology, designed for proteins tagged with the Cytiva Protein Select tag
- One-step purification workflow, combining affinity capture, traceless tag cleavage, and tag removal
- Protease-free process, with no protease required for tag cleavage
- High-flow agarose matrix, based on highly cross-linked agarose with an approximate 60 µm median particle size
- Protein ligand affinity, using a protein ligand derived from E. coli and coupled by epoxy chemistry
- Yield up to 20 mg protein/mL resin, depending on protein design and cleavage time
- Compatible with common aqueous buffers, supporting mild purification conditions for recombinant proteins
Benefits
- Simplifies purification for recombinant proteins that do not have an existing affinity binding partner.
- Helps obtain native, tag-free protein with no residual tag amino acids after cleavage.
- Reduces workflow complexity by replacing separate affinity capture, protease cleavage, and tag-removal steps.
- Provides a convenient prepacked column format for research and process development work.
- Supports a standardised affinity purification approach across diverse recombinant protein targets.
Why Choose the Cytiva Protein Select HiTrap Column, 5 x 5 mL (17542154)?
The Cytiva Protein Select HiTrap Column, 5 x 5 mL (17542154) is suited to laboratories that need a convenient prepacked format for Protein Select tag-based recombinant protein purification. John Morris Group supplies Cytiva chromatography columns and resins for Australian laboratories and can help confirm suitability for your purification workflow.
