The Cytiva Protein Select Resin, 500 mL (17542103), is a bulk affinity chromatography resin for purifying recombinant proteins using Cytiva Protein Select tag technology. It is designed for recombinant proteins that do not have an affinity binding partner, combining tagged protein capture, self-cleavage and tag removal in a single affinity workflow.
Key Features
- Bulk resin format, supplied as 500 mL for packing into suitable chromatography columns
- Self-cleaving affinity workflow, supporting purification and tag removal without adding a protease
- Highly cross-linked agarose matrix, providing strong pressure and flow properties for recombinant protein purification
- Specific tag affinity, with a protein ligand derived from E. coli immobilised to the resin matrix
- Approximate 60 µm particle size, based on median particle size of the cumulative volume distribution
- Yield up to 20 mg protein/mL resin, dependent on protein design and cleavage-time hold conditions
- High-flow operation, with recommended flow velocity of 450 to 600 cm/h and maximum flow velocity of 600 cm/h
- Operational pH range of 2 to 10, with compatibility in commonly used aqueous buffers
Benefits
- Helps simplify tagged recombinant protein purification by combining affinity capture, cleavage and tag removal in one workflow.
- Supports production of tag-free target protein without residual tag amino acids after cleavage.
- Reduces the need for separate protease cleavage and tag-removal steps compared with traditional tagged protein methods.
- Provides a platform affinity step for proteins that do not already have a convenient affinity binding partner.
- Supports scale-up work using the same resin chemistry across suitable packed-column formats.
Why Choose the Cytiva Protein Select Resin, 500 mL (17542103)?
Choose the Cytiva Protein Select Resin, 500 mL (17542103), when you need a bulk affinity resin for recombinant protein purification using the Cytiva Protein Select tag. John Morris Group supplies Cytiva chromatography products to Australian laboratories with local support for selection and ordering.
