The HiTrap Fibro PrismA Unit, 4-Pack (17549856), contains ready-to-use protein A fiber chromatography units for rapid cycling purification of monoclonal antibodies and Fc-containing recombinant proteins. Each pack includes four HiTrap Fibro PrismA units with 0.4 mL matrix volume per unit, supporting fast affinity chromatography cycles for research and process development applications.
Key Features
- 4-pack HiTrap Fibro PrismA format, supplied as four 0.4 mL protein A fiber chromatography units
- Derivatised electrospun cellulose fiber matrix, using an open pore structure where mass transfer is governed by convective flow
- PrismA protein A ligand, an alkali-stabilised ligand derived from E. coli and coupled by single-point attachment
- Rapid cycling chromatography performance, with typical HiTrap Fibro PrismA cycle times of approximately 3 min
- High binding capacity at short residence times, with approximately 30 mg IgG/mL matrix dynamic binding capacity
- Approximately 12 mg IgG per HiTrap unit, based on typical dynamic binding capacity per unit
- Recommended operating flow up to 16 mL/min, equivalent to 40 matrix volumes per minute for the HiTrap Fibro PrismA format
- Broad cleaning and operating range, with pH 3 to 12 operational stability and pH 2 to 14 CIP stability
Benefits
- Enables fast mAb purification cycles for clone selection, candidate screening, and early process development workflows.
- Helps reduce purification time compared with resin-based chromatography by using convective flow through the fiber matrix.
- Supports real-time UV, pH, and conductivity detection when connected to a compatible ÄKTA chromatography system.
- Allows repeated use with alkaline cleaning, helping reduce carryover risk between purification cycles.
Why Choose the HiTrap Fibro PrismA Unit, 4-Pack (17549856)?
Choose the HiTrap Fibro PrismA Unit, 4-Pack (17549856), when your workflow needs ready-to-use protein A fiber chromatography units for rapid mAb purification in research or process development. John Morris Group can support Australian laboratories with Cytiva chromatography product selection, ordering, and related workflow requirements.
